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slidehyb ii hybridization buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher slidehyb ii hybridization buffer
    Slidehyb Ii Hybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slidehyb+buffer/us11642396-191-12-16
    Average 86 stars, based on 1 article reviews
    slidehyb ii hybridization buffer - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Genomics-Based Molecular Epidemiology of Campylobacter jejuni Isolates from Feedlot Cattle and from People in Alberta, Canada
    Article Snippet: .. SlideHyb Buffer #2 (55 μl; Ambion, Austin, TX) was then added to the 20 μl tube from the quantification step and mixed gently, and the tube was placed in the heat block for 5 min (65 to 67°C). .. SlideHyb Buffer #2 (55 μl; Ambion, Austin, TX) was then added to the 20 μl tube from the quantification step and mixed gently, and the tube was placed in the heat block for 5 min (65 to 67°C).

    Comparison:

    Article Title: Mecp2 -Null Mice Provide New Neuronal Targets for Rett Syndrome
    Article Snippet: After degradation of template RNA, every labeled sample was purified using the Cyscribe GFX Purification kit (Amersham). .. Samples for comparison were mixed in pairs in order to carry out a direct competition hybridization experiment, then combined with SlideHyb Buffer (Ambion), mouse Cot-1 DNA (Invitrogen Corp.), RNA polyA (Sigma-Aldrich, St Louis, MO), and yeast tRNA (Invitrogen Corp.). ..

    Hybridization:

    Article Title: Mecp2 -Null Mice Provide New Neuronal Targets for Rett Syndrome
    Article Snippet: After degradation of template RNA, every labeled sample was purified using the Cyscribe GFX Purification kit (Amersham). .. Samples for comparison were mixed in pairs in order to carry out a direct competition hybridization experiment, then combined with SlideHyb Buffer (Ambion), mouse Cot-1 DNA (Invitrogen Corp.), RNA polyA (Sigma-Aldrich, St Louis, MO), and yeast tRNA (Invitrogen Corp.). ..

    Article Title: An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome
    Article Snippet: Then, Cy3 or Cy5 dye-esters (Amersham Pharmacia Biotech, Freiburg, Germany) in 4.5 μl DMSO (Fluka, Buchs, Switzerland) were added to the cDNA in 0.1 M Na 2 CO 3 and incubated in the dark at room temperature for 2 h. After purification on QIAquick columns, the concentration of the labeled cDNA and the dye incorporation rate were determined. .. For hybridization, the labeled cDNAs were taken up in SlideHyb buffer #1 (Ambion, Woodward, USA), denatured at 95°C for 5 min, and applied to the array. .. For hybridization, the labeled cDNAs were taken up in SlideHyb buffer #1 (Ambion, Woodward, USA), denatured at 95°C for 5 min, and applied to the array.

    Article Title: Gene expression analysis of bovine blastocysts produced by parthenogenic activation or fertilisation
    Article Snippet: The processes underlying the very first moments of embryonic development are still not well characterised in mammals.. To better define the kinetics of events taking place following fertilisation, it would be best to have perfect synchronisation of sperm entry.. With fertilisation occurring during a time interval of 6 to 12 h in the same group of fertilised oocytes, this causes a major variation in the time of activation of embryonic development.

    Yeast:

    Article Title: Mecp2 -Null Mice Provide New Neuronal Targets for Rett Syndrome
    Article Snippet: After degradation of template RNA, every labeled sample was purified using the Cyscribe GFX Purification kit (Amersham). .. Samples for comparison were mixed in pairs in order to carry out a direct competition hybridization experiment, then combined with SlideHyb Buffer (Ambion), mouse Cot-1 DNA (Invitrogen Corp.), RNA polyA (Sigma-Aldrich, St Louis, MO), and yeast tRNA (Invitrogen Corp.). ..

    other:

    Article Title: Complex patterns of copy number variation at sites of segmental duplications: an important category of structural variation in the human genome.
    Article Snippet: The structural diversity of the human genome is much higher than previously assumed although its full extent remains unknown.. To investigate the association between segmental duplications that display constitutive copy number diVerences (CNDs) between humans and the great apes and those which exhibit polymorphic copy number variations (CNVs) between humans, we analysed a BAC array enriched with segmental duplications displaying such CNDs.. This study documents for the Wrst time that in addition to human-speciWc gains common to all humans, these duplication clusters (DCs) also exhibit polymorphic CNVs > 40 kb.

    Article Title: Cytogenetic and cDNA Microarray Expression Analysis of MCF10A Human Breast Cancer Progression Cell Lines
    Article Snippet: Reference and test sample genomic DNA (1 μg each) were individually fluorescently labeled using the BioPrime DNA labeling kit (Invitrogen, Carlsbad, CA) for 18 h at 37°C with the appropriate Cy dye (Cy3 or Cy5).

    Article Title: Global analysis of host response to induction of a latent bacteriophage
    Article Snippet: Aminoallyl-labeled dUTP (Ambion) was included in the RT reaction mix in a 4:1 ratio of aa-dUTP:unlabeled dUTP.

    Labeling:

    Article Title: An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome
    Article Snippet: Then, Cy3 or Cy5 dye-esters (Amersham Pharmacia Biotech, Freiburg, Germany) in 4.5 μl DMSO (Fluka, Buchs, Switzerland) were added to the cDNA in 0.1 M Na 2 CO 3 and incubated in the dark at room temperature for 2 h. After purification on QIAquick columns, the concentration of the labeled cDNA and the dye incorporation rate were determined. .. For hybridization, the labeled cDNAs were taken up in SlideHyb buffer #1 (Ambion, Woodward, USA), denatured at 95°C for 5 min, and applied to the array. .. For hybridization, the labeled cDNAs were taken up in SlideHyb buffer #1 (Ambion, Woodward, USA), denatured at 95°C for 5 min, and applied to the array.

    Purification:

    Article Title: The Transcriptional Response to Encystation Stimuli in Giardia lamblia Is Restricted to a Small Set of Genes
    Article Snippet: For dual-channel microarray analysis, extracted total RNA was processed using an amino allyl MessageAmp II RNA amplification kit (Ambion, Austin, TX) and labeled with N -hydroxysuccinimidyl ester-derivatized reactive dye Cy3 or Cy5 according to the manufacturer's protocol. .. After purification, 2 μg each of Cy3- or Cy5-labeled RNA was denatured, added to SlideHyb buffer I (Ambion), and hybridized to G. lamblia version 2 microarrays (TIGR) in a Tecan HybStation at Functional Genomics Center Zurich, Switzerland. ..

    Functional Assay:

    Article Title: The Transcriptional Response to Encystation Stimuli in Giardia lamblia Is Restricted to a Small Set of Genes
    Article Snippet: For dual-channel microarray analysis, extracted total RNA was processed using an amino allyl MessageAmp II RNA amplification kit (Ambion, Austin, TX) and labeled with N -hydroxysuccinimidyl ester-derivatized reactive dye Cy3 or Cy5 according to the manufacturer's protocol. .. After purification, 2 μg each of Cy3- or Cy5-labeled RNA was denatured, added to SlideHyb buffer I (Ambion), and hybridized to G. lamblia version 2 microarrays (TIGR) in a Tecan HybStation at Functional Genomics Center Zurich, Switzerland. ..



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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Image Search Results


    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Journal: BMC Immunology

    Article Title: The gastrointestinal nematode Trichostrongylus colubriformis down-regulates immune gene expression in migratory cells in afferent lymph

    doi: 10.1186/1471-2172-11-51

    Figure Lengend Snippet: Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Article Snippet: Cy3 and Cy5-labelled cDNA were pooled, concentrated to 10 μl by ethanol precipitation prior to denaturation at 95°C, combining with SlideHyb glass array hybridisation buffer #1 (60 ul, Ambion) and applied to the array.

    Techniques: Infection, Hybridization